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Recombinant human MRPL15 protein

Cat:P04049

Summary:

【Derived From】: E.coli
【Endotoxin】: Not measured
【Amino Acid】: 1-297aa
【Purity】: ≥85% by SDS-PAGE.
【Name】: MRPL15
【Full Name】: mitochondrial ribosomal protein L15
【Uniprot】: Q9P015
【Gene ID】: 29088
【Species Reactivity】: Human Mouse (Bovine Chicken)
【Mol Mass】: 33kDa
【Application】: Immunology research
【Purification】: NI-NTA affinity purification
【Bioactive】: N0
【Tag】: With a 6×His tag at the N/C-terminus.
【Concentration】: 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】: Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.)
【Storage】: Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks, stored at < -20℃ for 1 year.
【Formulation】: Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion.Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit.They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed.Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA.Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology.This gene encodes a 39S subunit protein that belongs to the EcoL15 ribosomal protein family.A pseudogene corresponding to this gene is found on chromosome 15q.

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