Summary:
Recombinant human ALKBH4 protein
Cat：P04055
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 1-303aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： ALKBH4 |
| 【Full Name】： alkB，alkylation repair homolog 4 (E. coli) |
| 【Uniprot】： Q9NXW9 |
| 【Gene ID】： 54784 |
| 【Species Reactivity】： Human Mouse |
| 【Mol Mass】： 33kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
Dioxygenase that mediates demethylation of actin monomethylated at 'Lys-84' (K84me1), thereby acting as a regulator of actomyosin-processes (PubMed:23673617).Demethylation of actin K84me1 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration (PubMed:23673617).In addition to proteins, also demethylates DNA: specifically demethylates DNA methylated on the 6th position of adenine (N6-methyladenosine) DNA, thereby regulating Polycomb silencing (By similarity).
Verified picture
