Summary:
Recombinant human NMRAL1 protein
Cat：P04071
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 1-300aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： NMRAL1 |
| 【Full Name】： NmrA-like family domain containing 1 |
| 【Uniprot】： Q9HBL8 |
| 【Gene ID】： 57407 |
| 【Species Reactivity】： Human Mouse Rat (Bovine Chicken) |
| 【Mol Mass】： 33kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
Redox sensor protein.Undergoes restructuring and subcellular redistribution in response to changes in intracellular NADPH/NADP(+) levels.At low NADPH concentrations the protein is found mainly as a monomer, and binds argininosuccinate synthase (ASS1), the enzyme involved in nitric oxide synthesis.Association with ASS1 impairs its activity and reduces the production of nitric oxide, which subsecuently prevents apoptosis.Under normal NADPH concentrations, the protein is found as a dimer and hides the binding site for ASS1.The homodimer binds one molecule of NADPH.Has higher affinity for NADPH than for NADP(+).Binding to NADPH is necessary to form a stable dimer.
Verified picture
