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Recombinant human DDX50 protein

Cat:P04100

Summary:

【Derived From】: E.coli
【Endotoxin】: Not measured
【Amino Acid】: 1-350aa
【Purity】: ≥85% by SDS-PAGE.
【Name】: DDX50
【Full Name】: DEAD (Asp-Glu-Ala-Asp) box polypeptide 50
【Uniprot】: Q9BQ39
【Gene ID】: 79009
【Species Reactivity】: Human Mouse
【Mol Mass】: 39kDa
【Application】: Immunology research
【Purification】: NI-NTA affinity purification
【Bioactive】: No activity experimental data, to be verified.
【Tag】: With a 6×His tag at the N/C-terminus.
【Concentration】: 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】: Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 10μg/ml. Dissolve the lyophilized protein in distilled water.)
【Storage】: Reconstituted protein solution can be stored at 4-7℃ for 2-7 days, stored at < -20℃ for 1 year.
【Formulation】: Powder: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases.They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly.Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.This gene encodes a DEAD box enzyme that may be involved in ribosomal RNA synthesis or processing.This gene and DDX21, also called RH-II/GuA, have similar genomic structures and are in tandem orientation on chromosome 10, suggesting that the two genes arose by gene duplication in evolution.This gene has pseudogenes on chromosomes 2, 3 and 4.Alternative splicing of this gene generates multiple transcript variants, but the full length nature of all the other variants but one has not been defined.

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