Summary:
Recombinant human NMNAT2 protein
Cat：P04151
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 1-303aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： NMNAT2 |
| 【Full Name】： nicotinamide nucleotide adenylyltransferase 2 |
| 【Uniprot】： Q9BZQ4 |
| 【Gene ID】： 23057 |
| 【Species Reactivity】： Human Mouse Rat (Gorilla Bovine) |
| 【Mol Mass】： 33kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
Nicotinamide/nicotinate-nucleotide adenylyltransferase that acts as an axon maintenance factor (By similarity).Catalyzes the formation of NAD+from nicotinamide mononucleotide (NMN) and ATP.Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate but with a lower efficiency.Cannot use triazofurin monophosphate (TrMP) as substrate.Also catalyzes the reverse reaction, i.e.the pyrophosphorolytic cleavage of NAD+.For the pyrophosphorolytic activity prefers NAD+, NADH and NaAD as substrates and degrades nicotinic acid adenine dinucleotide phosphate (NHD) less effectively.Fails to cleave phosphorylated dinucleotides NADP+, NADPH and NaADP+.Axon survival factor required for the maintenance of healthy axons: acts by delaying Wallerian axon degeneration, an evolutionarily conserved process that drives the loss of damaged axons (By similarity).
Verified picture
