Summary:
Recombinant human TYR protein
Cat：P04159
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 1-377aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： TYR |
| 【Full Name】： tyrosinase (oculocutaneous albinism IA) |
| 【Uniprot】： P14679 |
| 【Gene ID】： 7299 |
| 【Species Reactivity】： Human Mouse (Chicken Bovine) |
| 【Mol Mass】： 41kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate.Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue.The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DNA double-strand breaks/DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation.Thereby, protects the transcription of many genes involved in neurological development and maintenance from the abortive activity of TOP2.Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DSBs due to DNA damage by radiation and free radicals.Has preference for single-stranded DNA or duplex DNA with a 4 base pair overhang as substrate.Acts as a regulator of ribosome biogenesis following stress.Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1.Constit
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