Summary:
Recombinant human APEX2 protein
Cat：P04178
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 172-521aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： APEX2 |
| 【Full Name】： APEX nuclease (apurinic/apyrimidinic endonuclease) 2 |
| 【Uniprot】： Q9UBZ4 |
| 【Gene ID】： 27301 |
| 【Species Reactivity】： Human Mouse (Bovine) |
| 【Mol Mass】： 39kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： No activity experimental data, to be verified. |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 10μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 2-7 days， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
Functions as a weak apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents.Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends.Displays also double-stranded DNA 3'-5' exonuclease, 3'-phosphodiesterase activities.Shows robust 3'-5' exonuclease activity on 3'-recessed heteroduplex DNA and is able to remove mismatched nucleotides preferentially.Shows fairly strong 3'-phosphodiesterase activity involved in the removal of 3'-damaged termini formed in DNA by oxidative agents.In the nucleus functions in the PCNA-dependent BER pathway.Required for somatic hypermutation (SHM) and DNA cleavage step of class switch recombination (CSR) of immunoglobulin genes.Required for proper cell cycle progression during proliferation of peripheral lymphocytes.
Verified picture
