Summary:
Recombinant human POLR2D protein
Cat：P04179
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 1-142aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： POLR2D |
| 【Full Name】： polymerase (RNA) II (DNA directed) polypeptide D |
| 【Uniprot】： O15514 |
| 【Gene ID】： 5433 |
| 【Species Reactivity】： Human Mouse |
| 【Mol Mass】： 16kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs.Pol II is the central component of the basal RNA polymerase II transcription machinery.It is composed of mobile elements that move relative to each other.RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element.The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft.The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA (By similarity).
Verified picture
