Summary:
Recombinant human NANS protein
Cat：P04251
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 1-360aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： NANS |
| 【Full Name】： N-acetylneuraminic acid synthase |
| 【Uniprot】： Q9NR45 |
| 【Gene ID】： 54187 |
| 【Species Reactivity】： Human Mouse |
| 【Mol Mass】： 40kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids.In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product.In insect cells, expression of this gene results in Neu5Ac and KDN production.This gene is related to the E.coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E.coli neuB-negative mutant.
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