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Recombinant human EIF4A1 protein

Cat:P04283

Summary:

【Derived From】: E.coli
【Endotoxin】: Not measured
【Amino Acid】: 1-360aa
【Purity】: ≥85% by SDS-PAGE.
【Name】: EIF4A1
【Full Name】: eukaryotic translation initiation factor 4A,isoform 1
【Uniprot】: P60842
【Gene ID】: 1973
【Species Reactivity】: Human Mouse (Gorilla Bovine Rabbit A.thaliana)
【Mol Mass】: 40kDa
【Application】: Immunology research
【Purification】: NI-NTA affinity purification
【Bioactive】: N0
【Tag】: With a 6×His tag at the N/C-terminus.
【Concentration】: 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】: Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.)
【Storage】: Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks, stored at < -20℃ for 1 year.
【Formulation】: Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

ATP-dependent RNA Humanelicase wHumanicHuman is a subunit of tHumane eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome.In tHumane current model of translation initiation, eIF4A unwinds RNA secondary structures in tHumane 5'-UTR of mRNAs wHumanicHuman is necessary to allow efficient binding of tHumane small ribosomal subunit, and subsequent scanning for tHumane initiator codon.

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