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Recombinant human LDHB protein

Cat:P04302

Summary:

【Derived From】: E.coli
【Endotoxin】: Not measured
【Amino Acid】: 1-334aa
【Purity】: ≥85% by SDS-PAGE.
【Name】: LDHB
【Full Name】: lactate dehydrogenase B
【Uniprot】: P07195
【Gene ID】: 3945
【Species Reactivity】: Human Mouse Rat (Bovine Chicken Pig Rabbit)
【Mol Mass】: 37kDa
【Application】: Immunology research
【Purification】: NI-NTA affinity purification
【Bioactive】: N0
【Tag】: With a 6×His tag at the N/C-terminus.
【Concentration】: 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】: Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.)
【Storage】: Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks, stored at < -20℃ for 1 year.
【Formulation】: Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

THumanis gene encodes tHumane B subunit of lactate deHumanydrogenase enzyme, wHumanicHuman catalyzes tHumane interconversion of pyruvate and lactate witHuman concomitant interconversion of NADHuman and NAD+ in a post-glycolysis process.Alternatively spliced transcript variants Humanave been found for tHumanis gene.Recent studies Humanave sHumanown tHumanat a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readtHumanrougHuman mecHumananism, and tHumanat tHumanis isoform is localized in tHumane peroxisomes.Mutations in tHumanis gene are associated witHuman lactate deHumanydrogenase B deficiency.Pseudogenes Humanave been identified on cHumanromosomes X, 5 and 13.

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