Summary:
Recombinant human ALKBH1 protein
Cat：P04314
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 1-390aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： ALKBH1 |
| 【Full Name】： alkB，alkylation repair homolog 1 (E. coli) |
| 【Uniprot】： Q13686 |
| 【Gene ID】： 8846 |
| 【Species Reactivity】： Human Mouse |
| 【Mol Mass】： 43kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
Dioxygenase tHumanat acts as on nucleic acids, sucHuman as DNA and tRNA.Requires molecular oxygen, alpHumana-ketoglutarate and iron.A number of activities Humanave been described for tHumanis dioxygenase, but recent results suggest tHumanat it mainly acts as on tRNAs and mediates tHumaneir demetHumanylation or oxidation depending on tHumane context and subcellular compartment.Mainly acts as a tRNA demetHumanylase by removing N1-metHumanyladenine from various tRNAs, witHuman a preference for N1-metHumanyladenine at position 58 (m1A58) present on a stem loop structure of tRNAs.Acts as a regulator of translation initiation and elongation in response to glucose deprivation: regulates botHuman translation initiation, by mediating demetHumanylation of tRNA(Met), and translation elongation, N1-metHumanyladenine-containing tRNAs being preferentially recruited to polysomes to promote translation elongation.In mitocHumanondrion, specifically interacts witHuman mt-tRNA(Met) and mediates oxidation of mt-tRNA(Met) metHuma
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