Summary:
Recombinant human SPC25 protein
Cat：P05966
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 1-224aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： SPC25 |
| 【Full Name】： SPC25，NDC80 kinetochore complex component，homolog (S. cerevisiae) |
| 【Uniprot】： Q9HBM1 |
| 【Gene ID】： 57405 |
| 【Species Reactivity】： Human Mouse Rat (Bovine Fruit fly Dog) |
| 【Mol Mass】： 26kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.
Verified picture
