Our endotoxin scavengers are designed to remove endotoxins from DNA, protein or other liquid samples. Under specific pH value, salt concentration and temperature conditions, endotoxin scavenger can specifically bind to DNA, recombinant protein and endotoxin in liquid samples. After high-speed centrifugation at room temperature, DNA or protein is retained in the water phase, while endotoxin is concentrated to a very small volume of the lower phase and removed. After more than 3 repeated extraction, the level of endotoxin with activity of 5000 ~ 50000EU/mL can be reduced to below 5 ~ 0.5EU/mL, that is, the reduction of 1000 ~ 10000 times.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
使用过程中DNA水相在下层,去除剂相在上层,请问去除剂最佳工作调件是什么?如何调整实验2022-07-19 13:38:31
Administrator1. E1040与样本混合后离心,上层为含DNA的水相下层为有机相。
2. 注意37℃水浴 5min后,需尽快离心。12000rpm 离心时,温度不宜低于30度。离心后及时吸取上清至新管中。
3. 若样本内毒素含量高可重复处理。具体步骤可见说明书。