BCIP/NBT Chromogen Kit
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Cat.No：PR1100 Solarbio
Storage：Store at -20℃，avoid light，6 months
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Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
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Spectrophotometer
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Shaker
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Product Information
| Name | BCIP/NBT Chromogen Kit |
| Storage | Store at -20℃，avoid light，6 months |
| Unit | Kit |
| Specification | 25ml 125ml |
Product Introduction:
NBT is Nitroblue tetrazolium chloride, a dark blue amorphous slightly soluble substance. BCIP, also known as 5-Bromo-4-Chloro-3-Indolyl Phosphate, is catalyzed by Alkaline phosphatase (AP).
BCIP is hydrolyzed to produce a highly reactive product that reacts with NBT to form an insoluble dark blue to bluish-purple precipitate.
Instructions for use:
1. Take 40ul 25×NBT and add it to 1ml 1×AP reaction buffer, mix it well, and then add 40ul 25×BCIP to mix it well, that is, to form a reaction working liquid. This reaction liquid is best used now. Note: Depending on the membrane size, the amounts of 1×AP reaction buffer, 25×BCIP, and 25×NBT can be scaled up equally.
2. Dip the washed PVDF/NC film into the reaction working liquid and gently shake it at room temperature for warming.
3. When the color depth of the protein band meets the requirements (generally about 20-30 minutes), the membrane is immersed in deionized water to stop the reaction.
Note:
BCIP and NBT are suspected carcinogens, please take necessary preventive measures. Wear gloves during operation, avoid contact with skin as much as possible, and rinse thoroughly after use.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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这个是Bcip/NBT碱性磷酸酶试剂盒吗 2023-05-24 17:04:39
Administrator您好，本试剂盒中的试剂在碱性磷酸酶的作用下生成不溶性的深蓝色至蓝紫色的沉淀。常用于检测碱性磷酸酶标记的抗体。