TMB Single-Component Substrate solution

Product Introduction:

At present, enzyme immunoassay (EIA) technology has been widely used in quantitative or qualitative detection of antigens, haptens or antibodies. Horseradish peroxidase (HRP) and its conjugates are commonly used in enzyme-linked immunoassay (ELISA). 3,3 ',5,5 '-tetramethylbenzidine (TMB) is widely used because of its higher sensitivity and non-carcinogenicity than other chromogens in HRP chromogenic reaction system. TMB is mainly used in enzyme-linked immunosorbent assay (ELISA), immune dot hybridization or immunohistochemistry, and chlorine and hydrogen peroxide detection analysis. In order to meet the development and production of different kit products and scientific research needs, the company has specially developed different types of TMB color developing solution for immunoanalysis based on HRP.

Product features:

Ready-to-use type: no mixing, convenient and fast, reduce error

High sensitivity: not lower than A, B liquid

Good stability: stored at 2-8℃, valid for not less than 36 months; The reading is stable after the color development terminates

Low background: The detection OD value of the substrate solution at 650nm is less than 0.04

Reliable quality: small differences between batches

Appearance: The reagent is generally colorless, sometimes slightly light blue or light yellow

Usage:

Use an appropriate clean container (rinse repeatedly with pure water), pour out an appropriate amount of one-component TMB color developing solution, and use it when it reaches room temperature.

Adding liquid: After adding HRP conjugate and incubating for a certain time, wash the plate with appropriate washing liquid 3-5 times, and add TMB color developing liquid 100ul per hole. According to the needs of individual experiments, incubate at room temperature (15-25℃) or 37℃ without light for 10-30 minutes or longer until the color is the expected shade.

Termination: Add 1M hydrochloric acid or sulfuric acid solution of equal volume to terminate the reaction, and the reaction liquid in the hole changes from blue to yellow.

Reading: The absorption value was measured at 450nm within 30 minutes after termination of the reaction.

Note: If a high reaction background or precipitation occurs, it indicates that the TMB substrate is reacting too strongly. In order to avoid precipitation, it can be read immediately after termination; Or further dilute the primary antibody and/or HRP conjugate.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.