Sodium Citrate Antigen Retrieval Solution,50×
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Cat.No：C1032 Solarbio
Storage：Store at RT，1 year
Appearance：Liquid
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> Protein and Immunity > Other Reagents > IHC > Sodium Citrate Antigen Retrieval Solution,50×
Product Information
| Name | Sodium Citrate Antigen Retrieval Solution，50× |
| Appearance | Liquid |
| Storage | Store at RT，1 year |
| Unit | Bottle |
| Specification | 100ml 500ml |
After the cell or tissue is fixed with paraformaldehyde, formaldehyde or other aldehydes, the intracellular antigens will form aldehyde bonds and carboxymethyl bonds, which will block part of the antigenic determinant. At the same time, the cross-linking between proteins will make the antigenic determinant hidden, resulting in weakened staining signal during immune staining, and even some false negative staining results. Therefore, when immunohistochemical staining is performed, antigen repair or exposure is required first, that is, the cross-linking formed between molecules during fixation is destroyed, and the original spatial form of the antigen is restored. So the detection rate of antigen can be improved, the background staining can be reduced, and the accuracy of detection can be improved.
Citrate Antigen Retrieval solution (50×) is a commonly used antigen repair solution, which can be used for the antigen repair of paraffin, frozen section and other samples fixed with paraformaldehyde, formaldehyde or other aldehydes. It can effectively remove the cross-linking between proteins caused by aldehydes fixing agents, and fully expose the epitopes in paraffin sections and other samples, thus greatly improving the effect of immunostaining.
Usually paraffin sections need to be treated with antigen repair, while frozen sections can not be treated with antigen repair. Antigen repair can improve the immunostaining effect of paraffin section and the staining effect of frozen section. When the immunostaining effect of frozen section is not satisfactory, antigen repair should be considered.
According to the calculation of 10ml antigen repair solution (1×) required for each film, 100ml antigen repair solution (50×) can be used for antigen repair of 500 samples.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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PH多少 2022-08-17 16:23:00
Administrator您好，50乘的PH大概是5.3，纯水稀释到工作浓度1乘的PH大概是6.0，工作液PH和您稀释用的水有关，另外PH测定也会受温度等条件影响会有一些波动