Product Introduction

The whole system of freeze-dried microspheres constant temperature amplification series of products makes the stability, ease of use and convenience of transportation of diagnostic reagents greatly improved. The whole system of constant temperature freeze-drying microspheres is prepared by a proprietary microsphere freeze-drying process, with uniform product type, non-crushing, long-term storage and avoiding cold chain transportation. For the development of diagnostic kits, the primer can be dried at the bottom of the EP reaction tube, and a freeze-dried microsphere can be added to become a freeze-dried product of the full amplification system, so the reagent performance is more stable, easy to store and transport.

The product is a whole system freeze-dried microsphere product, containing Low Salt, Mg2+, dNTP, Bst 4.0 DNA/RNA polymerase for constant temperature amplification. Since a large amount of H+ is generated during LAMP reaction, resulting in a decrease in pH of the reaction system, Ph-sensitive dyes can be used for visual LAMP detection under the condition of low buffer salt system. The product contains a Tris (pH8.8) buffer salt with a final concentration of 25μM(25 μl dissolved volume).

Precautions

(1) The red-yellow discoloration reaction depends on the change of pH in the reaction system, so Tris salt, NaOH and other components in the template have a crucial impact on the reaction. Elution with ddH2O is recommended when nucleic acid purified samples are tested.

(2) Special note on the use of DEPC water: Because DEPC treated ddH2O shows strong acidity, it will directly cause the lyophilized ball to become yellow after dissolution. Therefore, DEPC treated H2O must be adjusted by NaOH solution pH to 8.0-9.0 before use. We recommend direct use of 18.2ΩH2O prepared by the water cooler without affecting the amplification of the RNA sample.

(3) In the detection of crude samples, the best crude sample is swab margin sample, swab margin sample after ddH2O immersion, can directly use the immersion solution as a template for amplification, without nucleic acid purification step.

(4) Recommendations for LAMP finished reagents, Lyo Bst 4.0LS IsoAmp freeze-dried spheres and red pH indicator dye tubes are stable at room temperature and can be stored for a long time. After mixing the amplification primer with 10x red pH indicator dye (given in the product), drying at 70℃, and adding a freeze-dried ball can prepare the complete system detection reagent. Another method, the detection primer can be made into a freeze-dried ball with the liquid reagent, and added to the red pH indicator dye tube. People with development experience can optimise their own pH dyes to match the low-salt LAMP reaction freeze-dried microspheres.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.