Membrane/Cytoplasmic/Nuclear Protein Step Extraction Kit
Cat.No:EX1400 Solarbio
Storage:Store at 2-8℃,1 year
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Membrane/Cytoplasmic/Nuclear Protein Step Extraction KitStorage:Store at 2-8℃,1 year
Qty:
Size:
Storage | Store at 2-8℃,1 year |
Unit | Kit |
Specification | 50T 100T |
Membrane protein/cytoplasmic protein/nuclear protein extraction kit is suitable for step extraction of membrane protein/cytoplasmic protein/nuclear protein from various primary or passed cells and various solid tissues, such as brain, spinal cord, nerve junction or fiber, fat, liver, digestive tract, kidney, heart, muscle, blood vessel, connective tissue and other animal tissues. The extraction process is simple and convenient, and can be completed within 1 hour. This kit contains a unique formulation that effectively dissolves cell membrane components, including the plasma membrane, the nuclear membrane, and various organelle membranes. The kit contains a protease inhibitor mixture that prevents protease from degrading the protein and provides a guarantee for extracting high purity proteins. The protein extracted from this kit can be used for WesternBlotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gelshift gel retardation test, enzyme activity determination and other downstream protein research experiments. The protein extracted by this kit is an active protein with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Stiff substrate induces nucleus pulposus cell ferroptosis via YAP and N-cadherin mediated mechanotransduction
Click to check >>Author:Wencan Ke, Zhiwei Liao, Huaizhen Liang, Bide Tong, Yu Song, Gaocai Li, Liang Ma, Kun Wang, Xiaobo Feng, Shuai Li, Wenbin Hua, Bingjin Wang, Cao Yang
IF:11.0920
Publish_to:Advanced Healthcare Materials
PMID:37022980