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My CartReactivity：Rat,Mouse,Human
Application：WB,ELISA,IHC,IF
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：26986
Host：Rabbit
Qty：
Size：
| Name | Anti-PABPC1 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:1000-3000. IHC 1:50-200. IF 1:50-200. |
| Target | PABPC1 |
| Background | This gene encodes a poly(A) binding protein. The protein shuttles between the nucleus and cytoplasm and binds to the 3' poly(A) tail of eukaryotic messenger RNAs via RNA-recognition motifs. The binding of this protein to poly(A) promotes ribosome recruitment and translation initiation; it is also required for poly(A) shortening which is the first step in mRNA decay. The gene is part of a small gene family including three protein-coding genes and several pseudogenes. |
| Swiss Prot | P11940 |
| Gene ID | 26986 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | Recombinant protein of human PABPC1 |
| Public Immunogen Range | 1-270aa |
| Calculated MW | 61/71kDa |
| Observed MW | 75kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Y-Dilution Ratio | WB 1:1000-3000. IHC 1:50-200. IF 1:50-200. ELISA 1:8000-80000. |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Mouse Rat |
| Application | IF WB IHC ELISA |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with PABPC1 antibody diluted at 1:2000;Lane: Raw264.7,Neuro-2a,PC-12,A549,MCF7,HeLa,Mouse testis.
Immunohistochemistry of paraffin-embedded Human breast cancer with PABPC1 antibody diluted at 1:100.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with PABPC1 antibody diluted at 1:100.
Immunofluorescence analysis of A431 cells using PABPC1 antibody diluted at 1:100.
Immunofluorescence analysis of NIH3T3 cells using PABPC1 antibody diluted at 1:100.
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