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My CartReactivity：Mouse,Rat,Monkey,Human
Application：IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：28989
Host：Rabbit
Qty：
Size：
| Name | Anti-NTMT1 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Bovine;Zebrafish |
| Dilution Ratio | WB 1:1000-3000. IHC 1:50-200. |
| Target | NTMT1 |
| Background | METTL11A, also named N terminal RCC1 methyltransferase (NRMT), is an alpha N methyltransferase that methylates the N terminal amino group of target proteins containing the N terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved (1). It is responsible for N terminal methylation of RCC1, KLHL31, MYL2, MYL3, RB1, RPL23A and SET. NRMT lacks a SET domain but possesses a Rossman like a/b fold. The residues Asn169, Asp178, Asp181, and Ser183 of NRMT are important for substrate binding (2). RCC1 (Ran guanine nucleotide exchange factor) is the first protein for which any biological function of alpha N methylation by NRMT has been identified (3, 4). The multi spindle phenotype associated with either NRMT knockdown or methylation defective RCC1 mutants demonstrated the importance of alpha N methylation of RCC1 for normal bipolar spindle formation and chromosome aggregation (2). NRMT is robustly overexpressed in gastrointestinal cancers. |
| Swiss Prot | Q9BV86 |
| Gene ID | 28989 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | Recombinant protein of human NTMT1 |
| Public Immunogen Range | 1-224aa |
| Calculated MW | 16/25kDa |
| Observed MW | 19kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Monkey Rat Mouse |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with NTMT1 antibody diluted at 1:2000;Lane: U937,HeLa,SKOV3,Raji,293T,COS7,HepG2,Mouse testicle.
Immunohistochemistry of paraffin-embedded Human breast cancer with NTMT1 antibody diluted at 1:100.
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