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Determination of Significance：
Iron is one of the essential trace elements in human body. Fe2+ is the main component of hemoglobin, myoglobin, cytochrome and other enzyme systems, which could assist in the transport of oxygen and promote fat oxidation. Iron deficiency can easily cause anemia, metabolic disorders, and affect the immune function of the body.
Measurement Principle：
Fe2+ could react with Tripyridyltriazine to form a kind of blue compound under acid condition, which has an absorption peak at 593 nm. Changes of the absorbance at 593nm could be measured to reflect ferrous ion content.
Self Provided：
Spectrophotometer/microplate reader, balance, low temperature table centrifuge, constant temperature incubator/water bath, micro glass cuvette/96 well flat-bottom plate, pipette, mortar/homogenizer/cell ultrasonic crusher, ice, distilled water and concentrated sulfuric acid.
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