Determination of Significance：

Phytase, also known as inositol hexaphosphatase, is an enzyme that combines protein and sugar. Phytase can decompose phytic acid to produce inorganic phosphorus and inositol, greatly improving the utilization of nutrients by organisms. Soil phytase mainly comes from soil microorganisms and plays an important role in the phosphorus cycle. Soil phytase has a strong application prospect in the field of soil improvement and sustainable agricultural development.

Measurement Principle：

Under certain environmental conditions, soil phytase can decompose sodium phytate (twelve sodium inositol hexaphosphate) to produce inorganic phosphorus and inositol derivatives. Under acidic conditions, inorganic phosphorus reacts with ammonium molybdate chromogenic agent to produce blue molybdenum blue substance, which has a characteristic absorption peak at 700nm. The activity of soil phytase can be calculated by measuring the content of inorganic phosphorus.

Self Provided：

Spectrophotometer/Microplate Reader, Centrifuge, Water-bath/Constant Temperature Incubator, Transferpettor, Micro Glass Cuvette/96 Well Flat-bottom Plate, Mortar, Oscillator, Toluene, Concentrated Sulfuric acid, 30-50 Mesh Sieve , Ice and Distilled Water.

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