Sialic Acid Content Assay Kit

Determination of Significance：

Phytase, also known as inositol hexaphosphatase, is an enzyme that combines protein and sugar. Phytase can decompose phytic acid to produce inorganic phosphorus and inositol, greatly improving the utilization of nutrients by organisms. Soil phytase mainly comes from soil microorganisms and plays an important role in the phosphorus cycle. Soil phytase has a strong application prospect in the field of soil improvement and sustainable agricultural development.

Measurement Principle：

Under certain environmental conditions, soil phytase can decompose sodium phytate (twelve sodium inositol hexaphosphate) to produce inorganic phosphorus and inositol derivatives. Under acidic conditions, inorganic phosphorus reacts with ammonium molybdate chromogenic agent to produce blue molybdenum blue substance, which has a characteristic absorption peak at 700nm. The activity of soil phytase can be calculated by measuring the content of inorganic phosphorus.

Self Provided：

Spectrophotometer/Microplate Reader, Centrifuge, Water-bath/Constant Temperature Incubator, Transferpettor, Micro Glass Cuvette/96 Well Flat-bottom Plate, Mortar, Oscillator, Toluene, Concentrated Sulfuric acid, 30-50 Mesh Sieve , Ice and Distilled Water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.