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Protein transport inhibitor Kit

Storage:Store at -20℃,6 months.

Protein transport inhibitor Kit
Cat.No:
IK-PTI-1
Brand:
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
IK-PTI-1-2*100ul Inquiry Inquiry Inquiry Inquiry
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CAS号产品货号产品名称分子式分子量对应靶点产品描述
20350-15-6IB0270Brefeldin A布雷非德菌素 AC16H24O4280.36protein transport是一种内酯抗生素,是蛋白质运输的特定抑制剂,可以阻断分泌蛋白和膜蛋白从内质网向高尔基体的转运。也是一种自噬 (autophagy) 和 mitophagy 抑制剂。还是一种 CRISPR/Cas9 激动剂,可抑制 HSV-1病毒,并具有抗癌活性。
22373-78-0IM1050Monensin Sodium Salt莫能霉素钠C36H61NaO11692.85protein transportMonensin sodium salt 是一种抗生素。
本套装内含1000×的Brefeldin A(2mM in DMSO)、Monensin(10.6mM in EtOH)溶液各一支,每支溶液100μL。
Brefeldin A是一种蛋白质运输的特定抑制剂,可以阻断分泌蛋白和膜蛋白从内质网向高尔基体的转运,常用于抑制细胞因子等蛋白的分泌,并用于增强分泌蛋白的免疫染色检测。Monensin是一种常用的蛋白转运抑制剂,可破坏高尔基体,抑制细胞内的蛋白转运,常用于抑制细胞因子等分泌型蛋白的分泌,使其保留在细胞内。
PMA是一种有效的PKC激活剂;Ionomycin是一种有效的,选择性的钙离子载体。PMA常与Ionomycin联用,刺激免疫细胞活化,用于诱导体外细胞培养物产生细胞因子。
本套装适用于防止蛋白质转运至细胞外间隙的测定。将其添加至细胞培养基中可抑制胞内蛋白分泌/转运通路,从而导致内质网和高尔基体中出现分泌性蛋白积聚。可与细胞激活Kit-2(Cat:IK-CEA-2)联用,准确分析细胞因子的表达水平。


Remark:
These protocols are for reference only. Solarbio does not independently validate these methods.

Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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