Determination of Significance:
Carbonic anhydrase (CA, EC4.2.1.1) is a metalloenzyme with Zn2+ as the active center, which can efficiently catalyze the reversible hydration reaction of CO2: CO2+H2O ?HCO3-+H+ at a rate 107 times higher than that under natural conditions, which is one of the fastest known enzymes. It is one of the fastest known enzymes.
Measurement Principle:
Carbonic anhydrase can catalyze the reaction of p-nitrophenyl acetate to form p-nitrophenol, and the rate of increase of absorbance value at 405 nm reflects the activity of carbonic anhydrase.
Self Provided:
Spectrophotometer, centrifuge, water bath/incubator, adjustable pipette, 1mL glass cuvette, mortar/homogenizer/cell sonicator, ice and distilled water.
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