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My Cart> Antibodies > Secondary Antibodies > Purified Secondary Antibodies > Rabbit anti-Rat IgGReactivity：Rat
Application：WB,ELISA
Clone Type：Polyclonal Antibody
Appearance：Liquid
Storage：Shipped at 4℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles).
Host：Rabbit
Qty：
Size：
| Name | Rabbit Anti-Rat IgG（Purified Antibody） |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:1000-20000.;ELISA:1:5000-100000. |
| Background | Immunoglobulin G (IgG)，is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions;to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| Immunogen | Rat IgG full length protein |
| Calculated MW | 150kDa |
| Purification Method | Affinity purification |
| Storage Buffer | Buffer: PBS with 0.03% Proclin300（PH7.2-7.4). |
| Storage | Shipped at 4℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
| Appearance | Liquid |
| Concentration | 10mg/mL |
| Unit | Piece |
| Y-Application | ELISA WB |
| Y-Dilution Ratio | WB 1:1000-20000.;ELISA:1:5000-100000. |
| Specification | 1mg 10mg 100mg |
| Reactivity | Rat |
| Application | WB ELISA |
Product introduction：
The purified IgG antibody with high purity (>95%) and high activity was prepared by ammonium sulfate salting out and DEAE-ion-exchange cellulose chromatography, or batch adsorption purification. All kinds of purified antibodies are provided as liquid, which can be prepared in different concentrations according to user's requirements. It is best to store at -20℃ before use, and different concentrations can be diluted according to the use method. Usually different purposes can be diluted with the following buffer solution, pay attention to complete defrosting, fully mixed. Before use, it is best to remove the cold polymerized globulin and denatured protein by low temperature high-speed centrifugation, and then quantitate again.
Gold labeling: Diluted with pH9.0 borate buffer and labeled with 1-2 mg/ml.
HRP labeling: diluted with 0.01mol/L pH9.0, CB or coated solution CB, 6-8 mg/ml labeling
Fluorescent labeling: diluted with 0.5mol/L pH9.5, CB(carbonate buffer), 10-20 mg/ml labeling.
Biotinylated antibody: diluted with 0.1mol/L NaHCO3 and labeled with 1-2 mg/ml. Other methods, such as immunodiffusion, can be diluted with 0.01mol/L pH7.2 PBS and coated with 10-20 μg/ml ELISA at 4℃ overnight or 37℃ for 2 hours.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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