Basal gel is extracted from extracellular matrix protein-rich EHS mouse tumours to form a basement membrane matrix, whose main components are laminin, type IV collagen, nestin, heparin glycoprotein, and also contain growth factors and matrix metalloproteinases. At room temperature, the matrix gel polymerises to form a three-dimensional matrix with biological activity, simulating the structure, composition, physical properties and function of the cell basement membrane in vivo, which is conducive to in vitro cell culture and differentiation, and can be used for research on cell morphology, biochemical function, migration, invasion and gene expression.

Use: The cells can be directly coated without dilution, and the thickness of the coating can be 0.5 mm (thin layer) or 1.0 mm (thick layer).Matrigel can also be used to coat the surface of culture vessels after dilution in serum-free medium, and the concentration of Matrigel can be determined according to the type of cells and the purpose of the application.Matrigel can be formed into a gel at 10℃ or above, and in the course of the operation. Keep Matrigel on ice at all times during operation. All cell culture vessels, pipette tips, dispensing tubes, etc. that come into contact with Matrigel must be pre-cooled before use. The colour difference (yellowish to dark red) in the matrix is due to the interaction of phenol red and bicarbonate with CO2, and will be reduced by equilibration with 5% CO2. After freezing and thawing, gently shake the vial to disperse the Matrigel. All operations should be carried out in a sterile environment. The caps of the reagent bottles can be wiped with 70% ethanol and allowed to dry naturally. A pre-cooled pipette should be used to ensure that the Matrigel is homogenised. For dissolution, the Matrigel matrix can be dissolved on ice overnight at 4℃ in a refrigerator. Matrigel can be re-liquefied at 4℃ after 24-48h.

Applications:

Matrigel forms a three-dimensional culture matrix that promotes the adherence and differentiation of epithelial cells, hepatocytes, Sertoli cells, melanoma cells, vascular endothelial cells, thyroid cells and hair follicle cells. At the same time, the base gel can also affect the protein expression of mammary epithelial cells, support the neogenesis of peripheral nerves and the differentiation of bovine oviduct epithelial cells. Different formulations of substrates can meet different experimental requirements.