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My CartReactivity：Mouse
Application：IHC,IF
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：54631
Host：Rabbit
Qty：
Size：
| Name | Anti-Nephrin Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | IHC 1:1500-3000. IF 1:1500-3000. |
| Target | Nephrin |
| Background | Nephrin is a protein necessary for the proper functioning of the renal filtration barrier. The renal filtration barrier consists of fenestrated endothelial cells, the glomerular basement membrane, and thepodocytes of epithelial cells. Nephrin is a transmembrane protein that is a structural component of the slit diaphragm. They are present on the tips of the podocytes and maintain the normal relationship between the basement membrane and the podocytes of the epithelial cells.A defect in the gene for nephrin, NPHS1, is associated with congenital nephrotic syndrome of the Finnish typeand causes massive amounts of protein to be leaked into the urine, or proteinuria. Nephrin is also required for cardiovascular development. |
| Swiss Prot | Q9QZS7 |
| Gene ID | 54631 |
| Subcellular Location | Cell membrane |
| Immunogen | A synthetic peptide of mouse Nephrin |
| Calculated MW | 138kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，100 μg/ml BSA and 50% glycerol. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 100ul |
| Reactivity | Mouse |
| Application | IF IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Immunohistochemistry of paraffin-embedded mouse kidney with Nephrin antibody diluted at 1:3000.
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