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My CartReactivity：Mouse,Human,Rat
Application：IHC,WB,IF
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：114548
Host：Rabbit
Qty：
Size：
| Name | Anti-NLRP3 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-1000. IHC 1:200-400. IF 1:200-400. |
| Target | NLRP3 |
| Background | NALP3 is a component of the innate immune system that functions as a pathogen recognition receptor (PRR) that recognizes pathogen-associated molecular patterns (PAMPs). NALP3 together with the adaptor ASC protein PYCARD forms a caspase-1 activating complex known as the NALP3 inflammasome. NALP3 in the absence of activating signal is kept in an inactive state complexed with HSP90 and SGT1 in the cytoplasm. NALP3 inflammasome detects danger signals such as crystallineuric acid and extracellular ATP released by damaged cells. These signals release of HSP90 and SGT1 from and recruit ASC protein and caspase-1 to the inflammasome complex. |
| Swiss Prot | Q96P20 |
| Gene ID | 114548 |
| Subcellular Location | Cytoplasm |
| Immunogen | A synthetic peptide of human NLRP3 |
| Calculated MW | 118kDa |
| Observed MW | 110kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，100 μg/ml BSA and 50% glycerol. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 100ul |
| Reactivity | Mouse Human Rat |
| Application | IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with NLRP3 antibody diluted at 1:500;Lane: THP-1 RAW264.7 Mouse spleen+LPS Mouse thymus Rat spleen+LPS.
Immunohistochemistry of paraffin-embedded mouse spleen with NLRP3 antibody diluted at 1:300.
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