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My CartReactivity：Human,Mouse
Application：IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：23533
Host：Rabbit
Qty：
Size：
| Name | Anti-PIK3R5 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Chicken;Pig |
| Dilution Ratio | WB 1:2000-5000. IHC 1:25-100. |
| Target | PIK3R5 |
| Background | Phosphatidylinositol 3-kinases (PI3Ks) phosphorylate the inositol ring of phosphatidylinositol at the 3-prime position, and play important roles in cell growth, proliferation, differentiation, motility, survival and intracellular trafficking. The PI3Ks are divided into three classes: I, II and III, and only the class I PI3Ks are involved in oncogenesis. This gene encodes the 101 kD regulatory subunit of the class I PI3K gamma complex, which is a dimeric enzyme, consisting of a 110 kD catalytic subunit gamma and a regulatory subunit of either 55, 87 or 101 kD. This protein recruits the catalytic subunit from the cytosol to the plasma membrane through high-affinity interaction with G-beta-gamma proteins. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found. |
| Swiss Prot | Q8WYR1 |
| Gene ID | 23533 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | Recombinant protein of human PIK3R5 |
| Public Immunogen Range | 1-495aa |
| Calculated MW | 55/97kDa |
| Observed MW | 55kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Mouse |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with PIK3R5 antibody diluted at 1:3000;Lane: U251.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with PIK3R5 antibody diluted at 1:50.
Immunohistochemistry of paraffin-embedded Human pancreatic cancer with PIK3R5 antibody diluted at 1:50.
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