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My CartReactivity：Human
Application：IHC,WB,ELISA
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：1462
Host：Rabbit
Qty：
Size：
| Name | Anti-VCAN Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:2000-5000. IHC 1:25-100. |
| Target | VCAN |
| Background | This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Swiss Prot | P13611 |
| Gene ID | 1462 |
| Subcellular Location | Cytoplasm |
| Immunogen | A synthetic peptide of human VCAN |
| Public Immunogen Range | 2520-2570/3396aa |
| Calculated MW | 74/182/265/370/373kDa |
| Observed MW | 270kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Y-Dilution Ratio | WB 1:2000-5000. IHC 1:25-100. ELISA 1:20000-200000. |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human |
| Application | WB IHC ELISA |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with VCAN antibody diluted at 1:3000;Lane: PC-3M-IE8.
Immunohistochemistry of paraffin-embedded Human renal cancer with VCAN antibody diluted at 1:50.
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