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Malic Acid Content Assay kit

BC5495-241212Manual

Cat.No：BC5495 Solarbio

Detection Method：Spectrophotometer/Microplate Reader

Storage：Store at -20℃，6 months

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Product Information

Name	Malic Acid Content Assay kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at -20℃，6 months
Unit	Kit
Specification	100T/48S

Determination of Significance：

L-malic acid is an intermediate product of the tricarboxylic acid cycle and an important part of the malate-aspartic acid shuttle. Malate-aspartic acid shuttle is required for the reductive equivalent transmitochondrial membrane transport process of oxidative phosphorylation. In lower organisms, malic acid is converted to lactic acid in the process of malolactic fermentation, which also produces CO2. Malic acid is commonly used as an additive in the food and pharmaceutical industries, and quantitative analysis of malic acid is also vital in the production of beer, wine, cheese and fruit.

Measurement Principle：

Malate dehydrogenase catalyzes malic acid and NAD to form oxaloacetic acid, NADH and NH4+. Under 1-mPMS, WST-1 reacts with NADH to produce water-soluble Formazan. The maximum absorption peak at 450nm can be used to calculate malic acid content.

Self Provided：

Spectrophotometer/Microplate reader, mortar/homogenizer/cell ultrasonic crusher, centrifuge, constant temperature foster box/water-bath, centrifuge, micro quartz cuvette/96 well flat-bottom plate, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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