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Determination of Significance：
Carbonic Anhydrase (CA, EC4.2.1.1) is a metal enzyme with Zn2+ as the active center, which can be used to efficiently catalyze reversible hydration reaction of CO2: CO2+H20?HCO3-+H+, with a catalytic rate up to 107 times that of natural conditions, which is one of the fastest enzymes known so far.
Measurement Principle：
Carbonic anhydrase can catalyze the reaction of acetic acid to nitrophenyl ester to p-nitrophenol. The activity of carbonic anhydrase can be reflected by detecting the increase rate of absorption value at 405nm.
Self Provided：
Ultraviolet spectrophotometer/microplate reader, desk centrifuge, water-bath/ constant temperature incubator, adjustable pipette, micro quartz cuvette/96 well flat-bottom UV plate, 30-50 mesh sieve, Toluene, ice and distilled water.
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