Determination of Significance:
Pyruvate plays an important role in the metabolism of glucose, fatty acids and amino acids through acetyl CoA.
Measurement Principle:
At pH=7.5, pyruvate reacts with NADH catalyzed by LDH to produce NAD+ and lactic acid. Under 1-mPMS, WST-1 reacts with NADH to produce water-soluble Formazan. Pyruvate content can be calculated by detecting the absorption value at 450nm.
Self Provided:
Ultraviolet spectrophotometer/microplate reader, desk centrifuge, water-bath/ constant temperature incubator, adjustable pipette, micro quartz cuvette/96 well flat-bottom plate, ultrasonic cleaning instrument, 30-50 mesh sieve, ice and distilled water.
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