Yeast Inorganic Pyrophosphatase
Cat.No：R4010 Solarbio
Storage：Store at -20℃，3 years.
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Oscillating Mixing & Stirring & Dispersing Instrument
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> Molecular Biology > Molecular Cloning-Related Enzymes > Nucleic Acid Extraction Related Enzymes > Yeast Inorganic Pyrophosphatase
Product Information
| Name | Yeast Inorganic Pyrophosphatase |
| Storage | Store at -20℃，3 years. |
| Unit | Bottle |
| Specification | 20U |
Product Introduction
The sterile pyrophosphatase (yeast) was purified from the recombinant E.coli strain, carrying the ppa gene cloned from Saccharomycescerevisiae and the fusion gene containing peptides from Mycobacteriumxenopi GyrA. The pyrophosphatase catalyzes inorganic pyrophosphate hydrolysis to orthophosphate: P207-4 +H20→ 2HP04-2. In nucleic acid amplification experiments, it can release the inhibition of the generated inorganic pyrophosphate on the reaction system.
Application
1. Increase RNA production in reverse transcription reaction
2. Enhanced DNA replication
Precautions
(1) This enzyme is active in a variety of reaction buffers. Usually, this enzyme can be directly inserted in HDA amplification, LAMP amplification and other experiments.
(2) The amount of this enzyme needs to be optimized in different experiments, and the concentration is usually adjusted between 0.05 and 1U/ml.
(3) The optimal reaction temperature of the enzyme is 25℃, and it is active at 16~37℃, and the enzyme can be inactivated at 65℃for 10min.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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