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My Cart> Analytical Standards > Analytical Reference > 4-Hydroxy-3,5-dimethoxycinnamic acidCAS：530-59-6
Purity：HPLC≥98%
Molecular Formula：C11H12O5
Molecular Weight：224.21
Appearance：White to off-white powder
Storage：Store at 2-8℃，2 years.
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| CAS | 530-59-6 |
| Name | 4-Hydroxy-3，5-dimethoxycinnamic acid |
| Purity | HPLC≥98% |
| Molecular Formula | C11H12O5 |
| Molecular Weight | 224.21 |
| Appearance | White to off-white powder |
| Solubility | ≥5mg/mL in Ethanol |
| Storage | Store at 2-8℃，2 years. |
| EC | EINECS 208-487-3 |
| MDL | MFCD00004401 |
| SMILES | COC1=C(O)C(OC)=CC(/C=C/C(O)=O)=C1 |
| InChI | InChI=1S/C11H12O5/c1-15-8-5-7(3-4-10(12)13)6-9(16-2)11(8)14/h3-6，14H，1-2H3，(H，12，13)/b4-3+ |
| Note | The reserve liquid is recommended to be packaged and frozen to avoid repeated freezing and thawing (-20℃, 1 month); The diluted working liquid is recommended to be used and prepared immediately |
| Unit | Bottle |
| Specification | 20mg |
Examples of using this product（for reference only）
HPLC–MS
We used an HPLC system, which consisted of a binary solvent delivery system, an on-line degasser, an auto-sampler, a column temperature controller, and a diode array detector. Separation was conducted using a C18 column (5 μm, 150 mm × 4.6 mm), and the temperature was set to 40℃. The injection volume was 0.01 mL, and the flow rate was 1 mL/min. Gradient elution was conducted using a binary system consisting of 10 % formic acid in DW (A) and 10 % formic acid in acetonitrile (B). The utilized gradient was as follows: isocratic 1 % B for 6 min, 1% – 3% B for 4 min, 3 % – 15 % B for 20 min, 15 % – 40 % B for 20 min, 40 % – 90 % B for 5 min, 90 % B isocratic for 10 min, 90 % – 1 % B for 5 min, and 1 % B isocratic for 10 min. A re-equilibration operation followed. The UV detection wavelength was set to 280 nm.The voltages of the sample cone and the capillary were set to 17 and 3000 V for the positive ion mode and 22 and 2000 V for the negative ion mode.
References：
[1]Zhang,Junhong,Tian,et al.Isolation and identification of phenolic compounds in Chinese purple yam and evaluation of antioxidant activity[J].LWT-Food Science & Technology, 2018, 96:161-165.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Isolation and identification of phenolic compounds in Chinese purple yam and evaluation of antioxidant activity
Click to check >>Author：Junhong Zhang; Honglei Tian; Ping Zhan; Fangling D
IF：6.0000
Publish_to：LWT - Food Science and Technology
PMID：
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