Atractylenolide Ⅲ

Examples of using this product（for reference only）

UPLC-PDA

The levels of the main active ingredients of MSJZT were detected using a UPLC-PDA method. Chromatographic separation was performed on a UPLC system with a photodiode array detector (PDA) and C18 (100 mm×2.1 mm, 1.7μm ) column. The column temperature was set at 30℃. The mobile phase was composed of 0.1% phosphoric acid (A) and acetonitrile (B). The optimum adsorption and elution conditions were set as follows: 0–1.5 min, 95% B; 1.5–6 min, 95–80% B; 7–10 min, 80–60% B; 10–10.5 min, 50–35% B; 10.5–12 min, 35–30% B; 12–12.5 min, 30–20% B; 12.5–16 min, 20–10% B; 16–16.5 min, 10–0% B; and 16.5–18 min, 0–95% B.

References：

Nie S, Zhao Y, Qiu X, Wang W, Yao Y, Yi M, Wang D. Metabolomic Study on Nude Mice Models of Gastric Cancer Treated with Modified Si Jun Zi Tang via HILIC UHPLC-Q-TOF/MS Analysis. Evid Based Complement Alternat Med. 2019 Jun 23;2019:3817879. doi: 10.1155/2019/3817879. PMID: 31341492; PMCID: PMC6612382.

HPLC

The active components of ZZD were analyzed by LC AT-20 HPLC with double solvent pump high-pressure gradient system and SPD-20A photodiode array detector. The chromatography was performed on 5 lm particles, 250 × 4.6 mm, C18 column (35 ℃) at a flow rate of 1.0 mL/min and eluted with 0.1% formic acid aqueous solution (A) and 0.1% formic acid acetonitrile (B), and the gradient elution was 0–7 min, 23% B; 7–17 min, 23–80% B; 17–20 min, 80–100% B; 20–30 min, 100% B.

References：

Wen Y, Zhan Y, Tang S, Liu F, Wu R, Kong P, Li Q, Tang X. Zhizhu decoction alleviates slow transit constipation by regulating aryl hydrocarbon receptor through gut microbiota. Pharm Biol. 2023 Dec;61(1):111-124. doi: 10.1080/13880209.2022.2157020. Erratum in: Pharm Biol. 2023 Dec;61(1):1413. PMID: 36562308; PMCID: PMC9793913.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.