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My CartReactivity：Human,Mouse,Rat
Application：IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：29761
Host：Rabbit
Qty：
Size：
| Name | Anti-USP25 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:2000-5000. IHC 1:50-200. |
| Target | USP25 |
| Background | Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains.The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function. |
| Swiss Prot | Q9UHP3 |
| Gene ID | 29761 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | Recombinant protein of human USP25 |
| Public Immunogen Range | 1-451aa |
| Calculated MW | 122/126/130kDa |
| Observed MW | 133kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Mouse Rat |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with USP25 antibody diluted at 1:3000;Lane: HeLa,SKOV3,Raw264.7,Mouse testis,Mouse brain,Rat testis.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with USP25 antibody diluted at 1:100.
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