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My CartReactivity：Human,Mouse
Application：WB,IF,IHC
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：4520
Host：Rabbit
Qty：
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| Name | Anti-MTF1 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:2000-5000. IHC 1:25-100. IF 1:50-200. |
| Target | MTF1 |
| Background | Zinc-dependent transcriptional regulator of cellular adaption to conditions of exposure to heavy metals (PubMed:8065932).Binds to metal responsive elements (MRE) in promoters and activates the transcription of metallothionein genes like metallothionein-2/MT2A (PubMed:8065932).Also regulates the expression of metalloproteases in response to intracellular zinc and functions as a catabolic regulator of cartilages (By similarity). |
| Swiss Prot | Q14872 |
| Gene ID | 4520 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | Recombinant protein of human MTF1 |
| Public Immunogen Range | 404-754aa |
| Calculated MW | 81kDa |
| Observed MW | 60kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Mouse |
| Application | WB IHC IF |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with MTF1 antibody diluted at 1:3000;Lane: Jurkat,U-251,U-937,HMC-1,THP-1.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with MTF1 antibody diluted at 1:50.
Immunofluorescence analysis of A431 cells using MTF1 antibody diluted at 1:50.
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