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My CartReactivity：Human,Mouse
Application：WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：5426
Host：Rabbit
Qty：
Size：
| Name | Anti-POLE Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:1000-3000. |
| Target | POLE |
| Background | Catalytic component of the DNA polymerase epsilon complex (PubMed:10801849).Participates in chromosomal DNA replication (By similarity).Required during synthesis of the leading DNA strands at the replication fork, binds at/or near replication origins and moves along DNA with the replication fork (By similarity).Has 3'-5' proofreading exonuclease activity that corrects errors arising during DNA replication (By similarity).Involved in DNA synthesis during DNA repair (PubMed:20227374, PubMed:27573199).Along with DNA polymerase POLD1 and DNA polymerase POLK, has a role in excision repair (NER) synthesis following UV irradiation (PubMed:20227374). |
| Swiss Prot | Q07864 |
| Gene ID | 5426 |
| Subcellular Location | Nucleus Cell membrane |
| Immunogen | A synthetic peptide of human POLE |
| Public Immunogen Range | 360-410/2286aa |
| Calculated MW | 262kDa |
| Observed MW | 262kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Mouse |
| Application | WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with POLE antibody diluted at 1:2000;Lane: Jurkat,HMC1,HeLa,HEL,A549,MCF7.
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