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My CartReactivity：Mouse,Human
Application：IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：51234
Host：Rabbit
Qty：
Size：
| Name | Anti-EMC4 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Cow;Chimpanzee;Xenopus laevis;Xenopus tropicalis |
| Dilution Ratio | WB 1:1000-3000. IHC 1:50-200. |
| Target | EMC4 |
| Background | Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:30415835, PubMed:29809151, PubMed:29242231, PubMed:32459176, PubMed:32439656).Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues (PubMed:30415835, PubMed:29809151, PubMed:29242231).Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices (PubMed:30415835, PubMed:29809151).It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes (PubMed:29809151, PubMed:29242231).By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors (PubMed:30415835).By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable). |
| Swiss Prot | Q5J8M3 |
| Gene ID | 51234 |
| Subcellular Location | Cytoplasm |
| Immunogen | A synthetic peptide of human EMC4 |
| Public Immunogen Range | 20-70/183aa |
| Calculated MW | 15/17/20kDa |
| Observed MW | 19kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Mouse |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with EMC4 antibody diluted at 1:2000;Lane: 293T,HepG2,A549,HeLa,SH-SY5Y,THP-1,U937,HepG2,Jurkat.
Immunohistochemistry of paraffin-embedded Human stomach cancer with EMC4 antibody diluted at 1:100.
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