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My Cart> Biochemical Assay Kits > Other Biochemical Series > Other Series > Calcium test kit (methyl thymol Blue complexed copper microplate method)
Detection Method：O-Crephenolphthalein Complex Ketone Microplate Method
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| Detection Method | O-Crephenolphthalein Complex Ketone Microplate Method |
| Unit | Kit |
| Specification | 96T |
Product introduction
Calcium is a metal element, which is a silver-white crystal at room temperature. Animal bones, clam shells and egg shells all contain calcium carbonate. The detection of calcium content in living organisms is mainly achieved by detecting the concentration of calcium ions. At present, the commonly used dyes include o-cresolphthalide complex ketone (OCPC), azo arsenic Ⅲ, methyl thymol blue (MTB), etc.
The kit uses calcium ions in solution under alkaline conditions to combine with OCPC to form a purplish red complex, adding magnesium ion chelator to remove magnesium ion background interference, detecting the absorbance at 575nm by microplate reader, and calculating the total calcium content according to the formula; The advantages of this method are as follows: 1. It is a routine method of serum calcium detection recommended by the world; 2. Single reagent, easy to operate; 3. High sensitivity; 4. Stable color development 5. Small sample size.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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