The kit contains SF dye or Biotin to rapidly label all components of the antibody. The labeling process involves simply mixing the antibody and dye in the reaction buffer solution provided in the kit for a short incubation period. Super-n-stain dyes no longer react at the end of labeling, so no further purification steps are required. After labeling, about 4-6 molecular dyes are covalently bound to 1 molecule of antibody. Super-n-stain is a covalent label, so antibodies labeled with it can be used for polychromatic fluorescence staining without dye transfer between antibodies. The Super-n-stain label is compatible with NaN3, low concentrations of glycerol, Tris, and glycine. The miniature ultrafiltration centrifuge tube provided with the kit quickly removes incompatible small molecule antibody stabilizers prior to labeling. The standard Super-n-stain labeling step can be used for BSA or gelatin samples up to 4 times larger than IgG (ug). Select the kit size based on the amount of IgG you wish to label. The optimized labeling procedure is suitable for stabilizing IgG samples with excess protein or ascites. In this case, the kit size is selected based on the total amount of protein needed to label the antibody sample (IgG + stabilizer, or the total amount of protein in the ascites). The optimization step can also be used to label samples with IgG levels below the minimum range by adding stabilizer proteins to bring the total protein level to the kit's labeling range. The optimization procedure is not recommended for labeling natural antiserum or hybridoma cell line culture supernatants because the antibody content of these samples is very low relative to the total protein. When immunofluorescence is performed directly with fluorescently labeled antibodies, you may need to use higher concentrations of antibodies to achieve the intensity of staining similar to indirect immunofluorescence detection (primary antibody plus labeled secondary antibody). In internal tests, it was found that indirect immunofluorescence staining results were about three times as strong as direct immunofluorescence staining signals. The labelled secondary antibody can still be attached to a single antibody labelled using the Super-n-stain kit, so if multiple antibodies from the same species are used in polychromatic immunofluorescence staining, the secondary antibody cannot distinguish between an unlabelled primary antibody of the same species and a labelled primary antibody labelled using the Super-n-stain kit. Super-n-stain? labeled antibodies can be used as a tertiary stain after detection by conventional indirect immunofluorescence.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.