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My CartReactivity：Human
Application：WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：9968
Host：Rabbit
Qty：
Size：
| Name | Anti-MED12 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Chimpanzee;Zebrafish |
| Dilution Ratio | WB 1:1000-3000. |
| Target | MED12 |
| Background | Mediator of RNA polymerase II transcription subunit 12 (MED12)， also named Activator-recruited cofactor 240 kDa component， Thyroid hormone receptor-associated protein complex 230 kDa component. MED12 is a component of the Mediator complex， a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. Molecular weight of MED12 is about 240kd.This antibody is specific to MED12. |
| Swiss Prot | Q93074 |
| Gene ID | 9968 |
| Subcellular Location | Nucleus |
| Immunogen | A synthetic peptide of human MED12 |
| Public Immunogen Range | 1-51/2177aa |
| Calculated MW | 243kDa |
| Observed MW | 250kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human |
| Application | WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with MED12 antibody diluted at 1:2000;Lane: HepG2,HMC1,Jurkat.
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