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My CartReactivity：Mouse,Rat,Human
Application：WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：9039
Host：Rabbit
Qty：
Size：
| Name | Anti-UBA3 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Zebrafish;Fruit fly |
| Dilution Ratio | WB 1:1000-3000. |
| Target | UBA3 |
| Background | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes， or E1s， ubiquitin-conjugating enzymes， or E2s， and ubiquitin-protein ligases， or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1， an amyloid beta precursor protein binding protein， to form a heterodimer， and then the enzyme complex activates NEDD8， a ubiquitin-like protein， which regulates cell division， signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
| Swiss Prot | Q8TBC4 |
| Gene ID | 9039 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | Recombinant protein of human UBA3 |
| Public Immunogen Range | 116-445aa |
| Calculated MW | 50/52kDa |
| Observed MW | 55kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Mouse Rat Human |
| Application | WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with UBA3 antibody diluted at 1:2000;Lane: Mouse brain,Rat brain.
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