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My CartReactivity：Mouse,Rat,Human
Application：IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：1802
Host：Rabbit
Qty：
Size：
| Name | Anti-DPH2 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Bovine |
| Dilution Ratio | WB 1:2000-5000. IHC 1:25-100. |
| Target | DPH2 |
| Background | This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain， and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq， Jan 2016] |
| Swiss Prot | Q9BQC3 |
| Gene ID | 1802 |
| Subcellular Location | Cytoplasm |
| Immunogen | Recombinant protein of human DPH2 |
| Public Immunogen Range | 1-490aa |
| Calculated MW | 28/39/52kDa |
| Observed MW | 52kDa |
| Purification Method | Octanoic acid-ammonium sulfate precipitation |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Mouse Rat Human |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with DPH2 antibody diluted at 1:3000;Lane: HepG2,A549,HeLa,MCF7.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with DPH2 antibody diluted at 1:50.
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