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My CartReactivity：Mouse,Human
Application：IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：54778
Host：Rabbit
Qty：
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| Name | Anti-RNF111 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Gorilla |
| Dilution Ratio | WB 1:1000-3000. IHC 1:50-200. |
| Target | RNF111 |
| Background | The protein encoded by this gene is a nuclear RING-domain containing E3 ubiquitin ligase. This protein interacts with the transforming growth factor (TGF) -beta/NODAL signaling pathway by promoting the ubiquitination and proteosomal degradation of negative regulators， like SMAD proteins， and thereby enhances TGF-beta target-gene transcription. As a modulator of the nodal signaling cascade， this gene plays a critical role in the induction of mesoderm during embryonic development. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. |
| Swiss Prot | Q6ZNA4 |
| Gene ID | 54778 |
| Subcellular Location | Cytoplasm |
| Immunogen | Recombinant protein of human RNF111 |
| Public Immunogen Range | 1-137aa |
| Calculated MW | 108/109/110kDa |
| Observed MW | 105kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Mouse Human |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with RNF111 antibody diluted at 1:2000;Lane: HeLa,U-251MG,NIH3T3.
Immunohistochemistry of paraffin-embedded Human pancreatic cancer with RNF111 antibody diluted at 1:100.
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