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My CartReactivity：Human,Mouse,Rat
Application：IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：686
Host：Rabbit
Qty：
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| Name | Anti-BTD Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Bovine;Fruit fly |
| Dilution Ratio | WB 1:2000-5000. IHC 1:25-100. |
| Target | BTD |
| Background | The protein encoded by this gene functions to recycle protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine)， a normal product of carboxylase degradation， resulting in regeneration of free biotin. The encoded protein has also been shown to have biotinyl transferase activity. Mutations in this gene are associated with biotinidase deficiency. Multiple transcript variants encoding different isoforms have been described. |
| Swiss Prot | P43251 |
| Gene ID | 686 |
| Subcellular Location | Cytoplasm Cell membrane |
| Immunogen | Recombinant protein of human BTD |
| Public Immunogen Range | 195-544aa |
| Calculated MW | 59/61/62kDa |
| Observed MW | 63kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Mouse Rat |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with BTD antibody diluted at 1:3000;Lane: HeLa.
Immunohistochemistry of paraffin-embedded Human thyroid cancer with BTD antibody diluted at 1:50.
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