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My CartReactivity：Monkey,Human,Rat,Mouse
Application：IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：4659
Host：Rabbit
Qty：
Size：
| Name | Anti-PPP1R12A Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Zebrafish;Chicken |
| Dilution Ratio | WB 1:2000-5000. IHC 1:25-100. |
| Target | PPP1R12A |
| Background | Myosin phosphatase target subunit 1， which is also called the myosin-binding subunit of myosin phosphatase， is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation， which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound， active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase， which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase)， which is activated by GTP. RhoA， phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus， Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. |
| Swiss Prot | O14974 |
| Gene ID | 4659 |
| Subcellular Location | Cytoplasm Cell membrane |
| Immunogen | Recombinant protein of human PPP1R12A |
| Public Immunogen Range | 601-947aa |
| Calculated MW | 106/109/110/111/115kDa |
| Observed MW | 106kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Monkey Rat Mouse |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with PPP1R12A antibody diluted at 1:3000;Lane: K-562,Raji,MCF7,COS7,L-02,B16,HeLa.
Immunohistochemistry of paraffin-embedded Human breast cancer with PPP1R12A antibody diluted at 1:50.
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