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My CartReactivity：Rat,Human,Mouse
Application：IP,IHC,WB
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：57104
Host：Rabbit
Qty：
Size：
| Name | Anti-ATGL Monoclonal Antibody |
| Host | Rabbit |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-1000. IHC 1:50-100. IP 1:20. |
| Target | ATGL |
| Background | Adipose triglyceride lipase (ATGL) may function as a lipase and play a role in the adaptive response to a low energy state, such as fasting, by providing fatty acids to other tissues for oxidation. In addition, decreased expression of desnutrin in obesity models suggests its possible contribution to the pathophysiology of obesity. ATGL catalyzes the initial step in triglyceride hydrolysis in mammalian adipose tissue. ATGL contains a patatin domain common to plant acyl hydrolases. ATGL is highly expressed in adipose tissue of mice and humans. It exhibits high substrate specificity for triacylglycerol and is associated with lipid droplets. Inhibition of ATGL markedly decreases total adipose acyl hydrolase activity. Thus, ATGL and HSL coordinately catabolize stored triglycerides in adipose tissue of mammals. |
| Swiss Prot | Q96AD5 |
| Gene ID | 57104 |
| Subcellular Location | Cell membrane |
| Immunogen | Recombinant protein of human Adipose Triglyceride Lipase |
| Calculated MW | 55kDa |
| Observed MW | 55kDa |
| Purification Method | Affinity purification |
| Storage Buffer | 50mM Tris-Glycine(pH 7.4)，0.15M NaCl，40% Glycerol，0.01% Sodium azide and 0.05% BSA. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Human Mouse |
| Application | IP IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with ATGL antibody diluted at 1:1000;Lane: K562,C6,NIH3T3,HeLa.
Immunohistochemistry of paraffin-embedded Human breast cancer with ATGL antibody diluted at 1:100.
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